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plasmid phred102  (ATCC)


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    Structured Review

    ATCC plasmid phred102
    Plasmid Phred102, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+phred102/pHRed-102/10__1194_slash_jlr__m300105___jlr200-74-61-90
    Average 90 stars, based on 9 article reviews
    plasmid phred102 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Northern Blot:

    Article Title: Low-temperature effect on the sterol-dependent processing of SREBPs and transcription of related genes in HepG2 cells
    Article Snippet: .. DNA probes used in Northern blot analysis were obtained as follows: 1.4 kb probes for human SREBP-1a and SREBP-2 were released by restriction digestion of plasmids pCMV-CSA10 and pCMV-CS2 with Bam HI and Eco RI; the probe for HSS was a cDNA fragment (38); the probe for 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) was a 1.2 kb Hind III fragment from the plasmid pHRed102 (39); the probe for LDL receptor (LDLR) was a 0.9kb Xho I -Bgl II fragment obtained from a plasmid containing the full-length cDNA of human LDLR (pLDLR3, ATCC #57004); farnesyl diphosphate synthase (FPPS) probe was a 1.1 kb PCR product from rat FPPS cDNA; and the 1.2 kb probe for GAPDH was obtained by Bam HI -Sal I digestion of the human GAPDH cDNA. ..

    Plasmid Preparation:

    Article Title: Low-temperature effect on the sterol-dependent processing of SREBPs and transcription of related genes in HepG2 cells
    Article Snippet: .. DNA probes used in Northern blot analysis were obtained as follows: 1.4 kb probes for human SREBP-1a and SREBP-2 were released by restriction digestion of plasmids pCMV-CSA10 and pCMV-CS2 with Bam HI and Eco RI; the probe for HSS was a cDNA fragment (38); the probe for 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) was a 1.2 kb Hind III fragment from the plasmid pHRed102 (39); the probe for LDL receptor (LDLR) was a 0.9kb Xho I -Bgl II fragment obtained from a plasmid containing the full-length cDNA of human LDLR (pLDLR3, ATCC #57004); farnesyl diphosphate synthase (FPPS) probe was a 1.1 kb PCR product from rat FPPS cDNA; and the 1.2 kb probe for GAPDH was obtained by Bam HI -Sal I digestion of the human GAPDH cDNA. ..

    Polymerase Chain Reaction:

    Article Title: Low-temperature effect on the sterol-dependent processing of SREBPs and transcription of related genes in HepG2 cells
    Article Snippet: .. DNA probes used in Northern blot analysis were obtained as follows: 1.4 kb probes for human SREBP-1a and SREBP-2 were released by restriction digestion of plasmids pCMV-CSA10 and pCMV-CS2 with Bam HI and Eco RI; the probe for HSS was a cDNA fragment (38); the probe for 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) was a 1.2 kb Hind III fragment from the plasmid pHRed102 (39); the probe for LDL receptor (LDLR) was a 0.9kb Xho I -Bgl II fragment obtained from a plasmid containing the full-length cDNA of human LDLR (pLDLR3, ATCC #57004); farnesyl diphosphate synthase (FPPS) probe was a 1.1 kb PCR product from rat FPPS cDNA; and the 1.2 kb probe for GAPDH was obtained by Bam HI -Sal I digestion of the human GAPDH cDNA. ..



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    plasmid phred102  (ATCC)
    90
    ATCC plasmid phred102
    Plasmid Phred102, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+phred102/pHRed-102/10__1194_slash_jlr__m300105___jlr200-74-61-90
    Average 90 stars, based on 1 article reviews
    plasmid phred102 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

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